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6. Define all abbreviations (and don’t use excessive numbers of abbreviations).
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Abstracts must be in English and prepared strictly according to the submission guidelines. There is no limit on the number of abstracts submitted by a person as co-author (non-presenting author), but you can be presenting author for only one Abstract. The presenting author must register for the Congress by April 2, 2012, otherwise the abstract will be removed from the proceedings.
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Preliminary assessment of reproductive technologies in Wood Bison (Bison bison athabascae): implications for preserving genetic diversity
Jacob Thundathil1, Douglas Whiteside2, Brian Shea3, Darren Ludbrook4, Brett Elkin1,5, John Nishi6
1Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada T2N 4N1; 2Calgary Zoo Animal Health Centre, Calgary, AB, Canada; 3Alta Embryo Group, Calgary, AB, Canada; 4Bear Creek Animal Clinic, Grande Prairie, AB, Canada; 5Wildlife Division, Government of Northwest Territories, Yellowknife, NT, Canada; 6Government of Northwest Territories, Environment & Natural Resources, Fort Smith, NT, Canada.
High prevalence of bovine tuberculosis and brucellosis in free-ranging wood bison in the Canadian north poses a threat to nearby healthy bison populations, commercial bison and cattle ranches, and potentially to humans. There is a considerable impetus to salvage the genetics of infected bison and maintain a disease-free herd. In that regard, there is a great need to develop appropriate reproductive technologies. Therefore, the objective of this study was to develop a protocol to produce wood bison embryos (based on protocols used for cattle). Cumulus oocyte complexes (COC) aspirated from ovaries recovered after slaughter were matured in vitro, and fertilized with either frozen-thawed semen or chilled epididymal spermatozoa. Although both sources of spermatozoa resulted in acceptable rates of fertilization (64.4%, n = 45; 89.2%, n = 28, respectively) and cleavage (75.0%, n = 40; 92.5%, n = 40), production of morulae (7.5%, n = 40; 25.0%, n = 40) and blastocysts (7.5%, n = 40; 10.0%, n = 40) was low. To our knowledge, this is the first report regarding the in vitro production of bison embryos for genetic recovery of diseased wood bison. These techniques have substantial potential for conserving and managing the genetic diversity of wild bison, and may also have important management implications for genetic salvage of diseased bison populations in North America.